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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/15718
Title: 
Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
  • Vila Velha Univ Ctr
ISSN: 
1678-7757
Sponsorship: 
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Sponsorship Process Number: 
  • FAPESP: 08/08424-6
  • CNPq: 301029/2007-5
Abstract: 
This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. Methods: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as follows: in Group 1, no light-curing; in Groups 2, 3 and 4, the composite resin specimens were light-cured for 20, 40 or 60 s, respectively. In Group 5, filter paper discs soaked in 5 mu L PBS were used as negative controls. The resin specimens and paper discs were placed in wells of 24-well plates in which the odontoblast-like cells MDPC-23 (30,000 cells/cm(2)) were plated and incubated in a humidified incubator with 5% CO(2) and 95% air at 37 degrees C for 72 h. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). The data were analyzed statistically by Kruskal-Wallis and Mann-Whitney tests (p<0.05). Results: In G1, cell metabolism decreased by 86.2%, indicating a severe cytotoxicity of the non-light-cured composite resin. on the other hand, cell metabolism decreased by only 13.3% and 13.5% in G2 and G3, respectively. No cytotoxic effects were observed in G4 and G5. In G1, only a few round-shaped cells with short processes on their cytoplasmic membrane were observed. In the other experimental groups as well as in control group, a number of spindle-shaped cells with long cytoplasmic processes were found. Conclusion: Regardless of the photoactivation time used in the present investigation, the experimental composite resin presented mild to no toxic effects to the odontoblast-like MDPC-23 cells. However, intense cytotoxic effects occurred when no light-curing was performed.
Issue Date: 
1-Sep-2010
Citation: 
Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 5, p. 461-466, 2010.
Time Duration: 
461-466
Publisher: 
Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru
Keywords: 
  • Dental materials
  • Cytotoxicity
  • Dental pulp
  • Cultured cells
Source: 
http://dx.doi.org/10.1590/S1678-77572010000500006
URI: 
http://hdl.handle.net/11449/15718
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/15718
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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