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http://acervodigital.unesp.br/handle/11449/18940
- Title:
- Diagnosis of Giardia infections by PCR-based methods in children of an endemic area
- Universidade Estadual Paulista (UNESP)
- Universidade de São Paulo (USP)
- 1678-9199
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- FAPESP: 06/56151-3
- The present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit (R)) and polymerase chain reaction (PCR)-based methods. of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan and helminth infections, respectively. Concerning Giardia molecular diagnosis, from the 71 microscopy-positive samples, specific amplification of gdh and tpi fragments was noted in 68 (95.7%) and 64 (90%) samples, respectively. Amplification of gdh and tpi genes was observed, respectively, in 95.7% and 90% of microscopy-positive Giardia samples. For 144 microscopy-negative samples, gdh and tpi gene amplification products were obtained from 8.3% and 35.9% samples, respectively. The agreement between these genes was about 40%. The centrifuge-flotation based method was the most suitable means of Giardia diagnosis assessed in the present study by combining accuracy and low cost.
- 1-Jan-2011
- Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 2, p. 209-215, 2011.
- 209-215
- Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
- Giardia duodenalis
- children
- diagnosis
- PCR
- http://www.scielo.br/scielo.php?pid=S1678-91992011000200012&script=sci_arttext
- http://hdl.handle.net/11449/18940
- Acesso aberto
- outro
- http://repositorio.unesp.br/handle/11449/18940
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