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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/18979
Title: 
mRNA profile of Nellore calves after primary infection with Haemonchus placei
Author(s): 
Institution: 
  • Universidade Federal de São Carlos (UFSCar)
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
  • Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
  • Universidade Federal do Rio Grande do Norte (UFRN)
ISSN: 
0304-4017
Sponsorship: 
  • Prodetab
  • Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Abstract: 
Haemonchus parasites are responsible for many losses in animal production. However, few studies are available, especially of zebu cattle. In this study, we investigated mRNA differences of immune response genes in naive Nellore calves infected with Haemonchus placei, relating these differences to patterns of cellular infiltrate. Calves were infected with 15,000 H. placei 13 larvae and after 7 days lymph node and abomasum tissues were collected. IL-2, IL-4, IL-8, IL-12, IL-13, IFN-gamma, MCP-1, lysozyme, pepsinogen and TNF-alpha genes were evaluated by qPCR. Mast cells, eosinophils and globular leukocytes were counted by abomasum histology. In the infected group, IL-4, IL-13 and TNF-alpha were up-regulated in the abomasal lymph node. In the abomasum, IL-13 increased and TNF-alpha was down-regulated (p < 0.05). No differences were detected for mast cells and eosinophil counts in abomasal tissue (p > 0.05). We conclude that for this infection time, there was Th2 polarization but that cellular infiltrate in abomasal tissue takes longer to develop. (C) 2010 Elsevier B.V. All rights reserved.
Issue Date: 
10-Mar-2011
Citation: 
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 176, n. 2-3, p. 195-200, 2011.
Time Duration: 
195-200
Publisher: 
Elsevier B.V.
Keywords: 
  • Cytokines
  • Beef cattle
  • Haemonchus placei
  • Bos indicus
  • Gastrointestinal parasites
Source: 
http://dx.doi.org/10.1016/j.vetpar.2010.11.013
URI: 
http://hdl.handle.net/11449/18979
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/18979
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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