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http://acervodigital.unesp.br/handle/11449/36205
- Title:
- Crystallization and preliminary X-ray diffraction analysis of an anti-H(O) lectin from Lotus tetragonolobus seeds
- Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
- UFC
- Universidade Estadual Paulista (UNESP)
- 1744-3091
- The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour-diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 angstrom resolution using a synchrotron-radiation source. LTA crystals were found to be monoclinic, belonging to space group P2(1), with unit-cell parameters a = 68.89, b = 65.83, c = 102.53 angstrom, alpha = gamma = 90, beta = 92 degrees. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular-replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way.
- 1-Jul-2006
- Acta Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 680-683, 2006.
- 680-683
- Blackwell Publishing
- http://dx.doi.org/10.1107/S1744309106021312
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/36205 Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp
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