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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/36205
Title: 
Crystallization and preliminary X-ray diffraction analysis of an anti-H(O) lectin from Lotus tetragonolobus seeds
Author(s): 
Institution: 
  • Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
  • UFC
  • Universidade Estadual Paulista (UNESP)
ISSN: 
1744-3091
Abstract: 
The seed lectin from Lotus tetragonolobus (LTA) has been crystallized. The best crystals grew over several days and were obtained using the vapour-diffusion method at a constant temperature of 293 K. A complete structural data set was collected at 2.00 angstrom resolution using a synchrotron-radiation source. LTA crystals were found to be monoclinic, belonging to space group P2(1), with unit-cell parameters a = 68.89, b = 65.83, c = 102.53 angstrom, alpha = gamma = 90, beta = 92 degrees. Molecular replacement yielded a solution with a correlation coefficient and R factor of 34.4 and 51.6%, respectively. Preliminary analysis of the molecular-replacement solution indicates a new quaternary association in the LTA structure. Crystallographic refinement is under way.
Issue Date: 
1-Jul-2006
Citation: 
Acta Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 680-683, 2006.
Time Duration: 
680-683
Publisher: 
Blackwell Publishing
Source: 
http://dx.doi.org/10.1107/S1744309106021312
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/36205
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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