Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Miyata, Marcelo; Santos, Adolfo Carlos Barreto; Mendes, Natália Helena; Cunha, Eunice Atsuko; Melo, Fernando Augusto Fiúza de; Leite, Clarice Queico Fujimura

Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/7935

Title:

Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Author(s):

Institution:

Universidade Estadual Paulista (UNESP)
Laboratório Central de Saúde Pública
Instituto Clemente Ferreira

ISSN:

1517-8382

Sponsorship:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Abstract:

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

Issue Date:

1-Jun-2011

Citation:

Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.

Time Duration:

774-777

Publisher:

Sociedade Brasileira de Microbiologia

Keywords:

DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB

Source:

http://dx.doi.org/10.1590/S1517-83822011000200045

URI:

http://hdl.handle.net/11449/7935

Access Rights:

Acesso aberto

Type:

outro

Source:

http://repositorio.unesp.br/handle/11449/7935

Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Show full item record View Statistics