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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/112648
Title: 
Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
0003-9969
Abstract: 
Objectives: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).Design: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1 beta and TNF-alpha by ELISA.Results: The most effective concentration was 250 mg/mL and also promoted significant reduction (log(10)) in the biofilms of S. aureus (0.438 +/- 0.269), S. epiderrnidis (0.377 +/- 0.298), S. mutans (0.244 +/- 0.161) and C. albicans (0.746 +/- 0.209). Cell viability was similar to 100%. The production of IL-beta was similar to the control group (p > 0.05) and there was inhibition of TNF-alpha (p < 0.01).Conclusions: A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. (C) 2014 Elsevier Ltd. All rights reserved.
Issue Date: 
1-Aug-2014
Citation: 
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 8, p. 808-814, 2014.
Time Duration: 
808-814
Publisher: 
Elsevier B.V.
Keywords: 
  • Arctium lappa
  • Biofilms
  • Staphylococcus
  • Streptococcus mutans
  • Candida
  • RAW 264.7
Source: 
http://dx.doi.org/10.1016/j.archoralbio.2014.05.013
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/112648
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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