You are in the accessibility menu

Please use this identifier to cite or link to this item:
Reactivity of IgE to the allergen hyaluronidase from Polybia paulista (Hymenoptera, Vespidae) venom
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal do Rio Grande do Norte (UFRN)
  • Universidade Estadual de Campinas (UNICAMP)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Univ Estadual de Campinas-UNICAMP,
Sponsorship Process Number: 
  • FAPESP: 09/51539-1
  • Univ Estadual de Campinas-UNICAMP,2006/54810
To date, there are no allergenic extracts or components available in Brazil to diagnosis and treatment of patients with venom allergy from social wasp (Vespidae Family; Polistinae Subfamily) despite of the great number of existing species. We evaluated the immunogenic potential of the Hyal recombinant protein (Pp-Hyal-rec) which was expressed in an insoluble form in comparison with the allergenic native protein (Pp-Hyal-nat) for recognition of immunoglobulin E (IgE).in the serum of allergic patients to venom of the endemic social wasp Polybia paulista from Sao Paulo State, Brazil. Hyal cDNA from the venom of the social wasp P. paulista (Pp-Hyal) (GI: 302201582) was cloned into the expression vector pET-28a in Escherichia coli DE3 (BL21) cells. Solubilization and purification of Pp-Hyal-rec from inclusion bodies were performed using Ni2+ affinity chromatography (Ni-NTA-Agarose) under denaturing conditions. Both the native (Pp-Hyal-nat) and the recombinant (Pp-Hyal-rec) purified allergens were used for Western blotting to assess the levels of Pp-Hyal-IgE specific in the serum of 10 patients exclusively reactive to the venom of the social wasp P. paulista. The immune sera specifically recognized the band corresponding to the Pp-Hyal-rec protein (40 kDa) at a higher intensity than the native allergen (39 kDa). The sera recognized other proteins in P. paulista crude venom extract to a lesser extent, likely corresponding to other venom allergens such as phospholipase (34 kDa), Antigen 5 (25 kDa), and proteases. The recognition pattern of the immune sera to the Pp-Hyal-rec allergen strongly suggests that this recombinant antigen could be used for developing a diagnostic allergy test as well as for specific immunotherapy (IT). (C) 2014 Elsevier Ltd. All rights reserved.
Issue Date: 
Toxicon. Oxford: Pergamon-elsevier Science Ltd, v. 82, p. 104-111, 2014.
Time Duration: 
Elsevier B.V.
  • Polybia paulista
  • Venom
  • Allergen
  • Recombinant hyaluronidase
  • Immunoglobulin E (IgE)
  • Cross-immunoreactivity
Access Rights: 
Acesso restrito
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.