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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/11394
Título: 
Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test
Autor(es): 
Instituição: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
ISSN: 
0887-2333
Resumo: 
Epidemiological studies have provided evidence that high consumption of tomatoes effectively reduces the risk of reactive oxygen species (ROS)-mediated diseases such as cancer. Tomatoes are rich sources of lycopene, a potent singlet oxygen-quenching carotenoid. In addition to its antioxidant properties, lycopene shows an array of biological effects including antimutagenic and anticarcinogenic activities. In the present study, the chemopreventive action of lycopene was examined on DNA damage and clastogenic or aneugenic effects of H2O2 and n-nitrosodiethylamine (DEN) in the metabolically competent human hepatoma cell line (HepG2 cells). Lycopene at concentrations of 10. 25, and 50 mu M, was tested under three protocols: before, simultaneously, and after treatment with the mutagen, using the comet and micronucleus assays. Lycopene significantly reduced the genotoxicity and mutagenicity of H2O2 in all of the conditions tested. For DEN, significant reductions of primary DNA damage (comet assay) were detected when the carotenoid (all of the doses) was added in the cell culture medium before or simultaneously with the mutagen. In the micronucleus test, the protective effect of lycopene was observed only when added prior to DEN treatment. In conclusion, our results suggest that lycopene is a suitable agent for preventing chemically-induced DNA and chromosome damage. (C) 2007 Elsevier Ltd. All rights reserved.
Data de publicação: 
1-Mar-2008
Citação: 
Toxicology In Vitro. Oxford: Pergamon-Elsevier B.V. Ltd, v. 22, n. 2, p. 510-514, 2008.
Duração: 
510-514
Publicador: 
Pergamon-Elsevier B.V. Ltd
Palavras-chaves: 
  • lycopene
  • DNA damage
  • antigenotoxicity
  • antimutagenicity
  • HepG2 cells
Fonte: 
http://dx.doi.org/10.1016/j.tiv.2007.11.002
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/11394
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