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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/125581
Título: 
Effect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cells
Autor(es): 
Instituição: 
Universidade Estadual Paulista (UNESP)
ISSN: 
0300-5712
Resumo: 
To evaluate the effect of the oxidative stress on human dental pulp cells (HDPCs) promoted by toxic concentrations of hydrogen peroxide (H2O2) on its odontoblastic differentiation capability through time. Methods HDPCs were exposed to two different concentrations of H2O2 (0.1 and 0.3 μg/ml) for 30 min. Thereafter, cell viability (MTT assay) and oxidative stress generation (H2DCFDA fluorescence assay) were immediately evaluated. Data were compared with those for alkaline phosphatase (ALP) activity (thymolphthalein assay) and mineralized nodule deposition (alizarin red) by HDPCs cultured for 7 days in osteogenic medium. Results A significant reduction in cell viability and oxidative stress generation occurred in the H2O2-treated cells when compared with negative controls (no treatment), in a concentration-dependent fashion. Seven days after H2O2 treatment, the cells showed significant reduction in ALP activity compared with negative control and no mineralized nodule deposition. Conclusion Both concentrations of H2O2 were toxic to the cells, causing intense cellular oxidative stress, which interfered with the odontogenic differentiation capability of the HDPCs. Clinical significance The intense oxidative stress on HDPCs mediated by H2O2 at toxic concentrations promotes intense reduction on odontoblastic differentiation capability in a 7-day evaluation period, which may alter the initial pulp healing capability in the in vivo situation.
Data de publicação: 
2015
Citação: 
Journal of Dentistry, v. 43, n. 6, p. 750-756, 2015.
Duração: 
750-756
Palavras-chaves: 
  • Pulp cells
  • Hydrogen peroxide
Fonte: 
http://www.sciencedirect.com/science/article/pii/S0300571214003443
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/125581
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