You are in the accessibility menu

Please use this identifier to cite or link to this item:
Micro-arc oxidation as a tool to develop multifunctional calcium-rich surfaces for dental implant applications
  • Universidade Estadual Paulista (UNESP)
  • Universidade do Minho
  • Universidade Federal Fluminense (UFF)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
  • Fundação para a Ciência e a Tecnologia (FCT)
Sponsorship Process Number: 
  • FAPESP: 2012/03633-1
  • CNPq: 490761/2013-5
  • CNPq/PROMETRO: 563165/2010-3F
  • SFRH/BD: 70857/2010
  • PTDC/CTM: 68160/2006
  • PTDC/CTM: 67500/2006
  • CAPES/PVE: 1317/11-3
Titanium (Ti) is commonly used in dental implant applications. Surface modification strategies are being followed in last years in order to build Ti oxide-based surfaces that can fulfill, simultaneously, the following requirements: induced cell attachment and adhesion, while providing a superior corrosion and tribocorrosion performance. In this work micro-arc oxidation (MAO) was used as a tool for the growth of a nanostructured bioactive titanium oxide layer aimed to enhance cell attachment and adhesion for dental implant applications. Characterization of the surfaces was performed, in terms of morphology, topography, chemical composition and crystalline structure. Primary human osteoblast adhesion on the developed surfaces was investigated in detail by electronic and atomic force microscopy as well as immunocytochemistry. Also an investigation on the early cytokine production was performed. Results show that a relatively thick hybrid and graded oxide layer was produced on the Ti surface, being constituted by a mixture of anatase, rutile and amorphous phases where calcium (Ca) and phosphorous (P) were incorporated. An outermost nanometric-thick amorphous oxide layer rich in Ca was present in the film. This amorphous layer, rich in Ca, improved fibroblast viability and metabolic activity as well as osteoblast adhesion. High-resolution techniques allowed to understand that osteoblasts adhered less in the crystalline-rich regions while they preferentially adhere and spread over in the Ca-rich amorphous oxide layer. Also, these surfaces induce higher amounts of IFN-γ cytokine secretion, which is known to regulate inflammatory responses, bone microarchitecture as well as cytoskeleton reorganization and cellular spreading. These surfaces are promising in the context of dental implants, since they might lead to faster osseointegration.
Issue Date: 
Materials Science & Engineering. C, Biomimetic Materials, Sensors and Systems, v. 54, p. 196-206, 2015.
Time Duration: 
  • Micro-arc-oxidation
  • Calcium
  • Phosphorus
  • Titanium
  • Human bone-derived cells
  • Adhesion
  • Biointerphase
Access Rights: 
Acesso restrito
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.