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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/12711
Title: 
Viable human buccal mucosa cells do not yield typical nucleoids: Impacts on the single-cell gel electrophoresis/comet assay
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
0893-6692
Abstract: 
Buccal mucosa (BM) cells have been used in human biomonitoring studies for detecting DNA adducts and chromosomal damage in an epithelial cell population. In the present study, we have investigated if human BM cells are suitable for use in the single-cell gel electrophoresis (SCGE)/Comet assay as an approach for estimating the exposure of epithelial cells to DNA-damaging agents. Our results indicate that only a few cells from BM cell samples yield comets that can be analyzed by current methods, and that the yield of cells with comets is independent of the percentage of viable BM cells in the sample. Data generated after enzymatic enrichment of viable cells and immunomagnetic separation of epithelial cells suggest that most of the BM cells that do form comets are probably leukocytes. Moreover, by reevaluating specific cells after running the Comet assay, we found that viable epithelial BM cells give rise to atypical comets that are not included in the analysis. Comparing DNA migration patterns between small groups of smokers and nonsmokers indicated that long-term smoking had no effect on the subpopulation of cells that yield typical comets. Our results indicate that the SCGE assay, as it is commonly performed, may not be useful for genotoxicity monitoring in human epithelial BM cells.
Issue Date: 
1-Mar-2006
Citation: 
Environmental and Molecular Mutagenesis. Hoboken: Wiley-liss, v. 47, n. 2, p. 117-126, 2006.
Time Duration: 
117-126
Publisher: 
Wiley-Blackwell
Keywords: 
  • single-cell gel electrophoresis assay
  • DNA damage
  • viability
  • smoking
  • genotoxicity
  • cytotoxicity
Source: 
http://dx.doi.org/10.1002/em.20174
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/12711
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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