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http://acervodigital.unesp.br/handle/11449/129847
- Title:
- Rapid turbidimetric assay to potency evaluation of tigecycline in lyophilized powder
- Universidade Federal de Alfenas (UNIFAL)
- Universidade Estadual Paulista (UNESP)
- 0167-7012
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- FAPESP: 2009/53434-2
- Tigecycline, a first-in-class glycylcycline and an analog of the semisynthetic antibiotic minocycline, is a potent, broad-spectrum antibiotic that acts by the inhibition of protein translation in bacteria. This glycylcycline inhibits Gram-positive, Gram-negative, atypical, anaerobic and antibiotic-resistant organisms. There is no microbiological analytical method for tigecycline in lyophilized powder reported yet. Thus, this paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric method to quantify tigecycline in lyophilized powder, using Staphylococcus aureus as microorganism test and 3 x 3 parallel line assay design, with twenty tubes for each assay. The validated method showed good results of linearity in the concentration range from 3 to 432 mu g/mL (r(2) = 0.9999), selectivity, precision, robustness and accuracy of 99.74%. The results demonstrated the validity of the proposed bioassay, which allows reliable quantitation of tigecydine in pharmaceutical samples and therefore can be used as a useful alternative methodology for the routine quality control of this medicine. (C) 2015 Elsevier B.V. All rights reserved.
- 1-Mar-2015
- Journal Of Microbiological Methods. Amsterdam: Elsevier Science Bv, v. 110, p. 49-53, 2015.
- 49-53
- Elsevier B.V.
- Tigecycline
- Turbidimetric assay
- Quality control
- http://www.sciencedirect.com/science/article/pii/S0167701215000317
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/129847
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