Please use this identifier to cite or link to this item:
- Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale
- Universidade do Contestado (UnC)
- Universidade Estadual Paulista (UNESP)
- An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
- Veterinary Parasitology, v. 212, n. 3-4, p. 435-438, 2015.
- Elsevier B. V.
- Elisa test
- Serological diagnosis
- Acesso restrito
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.