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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/141083
Título: 
The use of real-time PCR (qPCR) for the diagnosis of bovine herpes virus 5 in formalin-fixed, paraffin-embedded bovine brain samples identified as bovine unspecific encephalitis
Autor(es): 
Instituição: 
  • Universidade Estadual Paulista (UNESP)
  • Universidad de Caldas
ISSN: 
2157-7579
Resumo: 
Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.
Data de publicação: 
2013
Citação: 
Journal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013.
Duração: 
1-5
Palavras-chaves: 
  • Bovine herpes virus 5
  • Real-time PCR
  • Diagnosis
  • BoHV-5
Fonte: 
http://dx.doi.org/10.4172/2157-7579.1000145
Endereço permanente: 
Direitos de acesso: 
Acesso aberto
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/141083
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