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- Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats
- Universidade Estadual Paulista (UNESP)
- Med Univ S Carolina
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- National Institutes of Health - National Institute of Dental and Craniofacial Research (NIH/NIDCR)
- CAPES: 4638-05
- NIH/NIDCR: 1R01DE018290
- NIH/NIDCR: P20RR017696
- Curcumin is a plant-derived dietary spice ascribed various biological activities. Curcumin therapeutic applications have been studied in a variety of conditions, but not on periodontal disease. Periodontal disease is a chronic inflammatory condition initiated by an immune response to micro-organisms of the dental biofilm. Experimental periodontal disease was induced in rats by injecting LPS in the gingival tissues on the palatal aspect of upper first molars (30 mu g LPS, 3 times/week for 2 weeks). Curcumin was administered to rats daily via oral gavage at 30 and 100 mg/kg body weight. Reverse transcriptase-qPCR and ELISA were used to determine the expression of IL-6, TNF-alpha and prostaglandin E-2 synthase on the gingival tissues. The inflammatory status was evaluated by stereometric and descriptive analysis on hematoxylin/eosin-stained sections, whereas modulation of p38 MAPK and NK-kappa B signaling was assessed by Western blot. Curcumin effectively inhibited cytokine gene expression at mRNA and protein levels, but NF-kappa B was inhibited only with the lower dose of curcumin, whereas p38 MAPK activation was not affected. Curcumin produced a significant reduction on the inflammatory infiltrate and increased collagen content and fibroblastic cell numbers. Curcumin potently inhibits innate immune responses associated with periodontal disease, suggesting a therapeutic potential in this chronic inflammatory condition.
- Innate Immunity. London: Sage Publications Ltd, v. 18, n. 1, p. 155-163, 2012.
- Sage Publications Ltd
- innate immunity
- periodontal disease
- Acesso restrito
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