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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/15930
Title: 
Quantitation of malondialdehyde in gingival crevicular fluid by a high-performance liquid chromatography-based method
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
ISSN: 
0003-2697
Sponsorship: 
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Sponsorship Process Number: 
FAPESP: 07/08362-8
Abstract: 
Lipid peroxidation (LPO) has been associated with periodontal disease, and the evaluation of malondialdehyde (MDA) in the gingival crevicular fluid (GCF), an inflammatory exudate from the surrounding tissue of the periodontium, may be useful to clarify the role of LPO in the pathogenesis of periodontal disease. We describe the validation of a method to measure MDA in the GCF using high-performance liquid chromatography. MDA calibration curves were prepared with phosphate-buffered solution spiked with increasing known concentrations of MDA. Healthy and diseased GCF samples were collected from the same patient to avoid interindividual variability. MDA response was linear in the range measured, and excellent agreement was observed between added and detected concentrations of MDA. Samples' intra- and interday coefficients of variation were below 6.3% and 12.4%, respectively. The limit of quantitation (signal/noise = 5) was 0.03 mu M. When the validated method was applied to the GCF, excellent agreement was observed in the MDA quantitation from healthy and diseased sites, and diseased sites presented more MDA than healthy sites (P < 0.05). In this study, a validated method for MDA quantitation in GCF was established with satisfactory sensitivity, precision, and accuracy. (C) 2012 Elsevier B.V. All rights reserved.
Issue Date: 
1-Apr-2012
Citation: 
Analytical Biochemistry. San Diego: Academic Press Inc. Elsevier B.V., v. 423, n. 1, p. 141-146, 2012.
Time Duration: 
141-146
Publisher: 
Academic Press Inc. Elsevier B.V.
Keywords: 
  • Lipid peroxidation
  • Malondialdehyde
  • Periodontal diseases
  • Gingival crevicular fluid
  • HPLC
Source: 
http://dx.doi.org/10.1016/j.ab.2012.01.016
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/15930
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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