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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/16018
Title: 
PDGF-B gene therapy accelerates bone engineering and oral implant osseointegration
Author(s): 
Institution: 
  • University of Michigan
  • Seoul Natl Univ
  • Universidade Estadual Paulista (UNESP)
  • Univ Milan
  • Tissue Repair Co
ISSN: 
0969-7128
Sponsorship: 
  • National Institutes of Health (NIH)/National Institute of Dental and Craniofacial Research (NIDCR)
  • AO Foundation Research Advisory Council
Sponsorship Process Number: 
NIH: R01-DE13397
Abstract: 
Platelet-derived growth factor-BB (PDGF-BB) stimulates repair of healing-impaired chronic wounds such as diabetic ulcers and periodontal lesions. However, limitations in predictability of tissue regeneration occur due, in part, to transient growth factor bioavailability in vivo. Here, we report that gene delivery of PDGF-B stimulates repair of oral implant extraction socket defects. Alveolar ridge defects were created in rats and were treated at the time of titanium implant installation with a collagen matrix containing an adenoviral (Ad) vector encoding PDGF-B (5.5 x 10(8) or 5.5 x 10(9) pfu ml (1)), Ad encoding luciferase (Ad-Luc; 5.5 x 10(9) pfu ml (1); control) or recombinant human PDGF-BB protein (rhPDGF-BB, 0.3 mg ml (1)). Bone repair and osseointegration were measured through backscattered scanning electron microscopy, histomorphometry, microcomputed tomography and biomechanical assessments. Furthermore, a panel of local and systemic safety assessments was performed. Results indicated that bone repair was accelerated by Ad-PDGF-B and rhPDGF-BB delivery compared with Ad-Luc, with the high dose of Ad-PDGF-B more effective than the low dose. No significant dissemination of the vector construct or alteration of systemic parameters was noted. In summary, gene delivery of Ad-PDGF-B shows regenerative and safety capabilities for bone tissue engineering and osseointegration in alveolar bone defects comparable with rhPDGF-BB protein delivery in vivo. Gene Therapy (2010) 17, 95-104; doi: 10.1038/gt.2009.117; published online 10 September 2009
Issue Date: 
1-Jan-2010
Citation: 
Gene Therapy. London: Nature Publishing Group, v. 17, n. 1, p. 95-104, 2010.
Time Duration: 
95-104
Publisher: 
Nature Publishing Group
Keywords: 
  • dental implant
  • platelet-derived growth factor
  • regenerative medicine
  • virus delivery
Source: 
http://dx.doi.org/10.1038/gt.2009.117
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/16018
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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