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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/16026
Title: 
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
1516-3725
Sponsorship: 
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Sponsorship Process Number: 
  • FAPESP: 03/10424-0
  • FAPESP: 05/03231-7
  • FAPESP: 05/04553-8
  • FAPESP: 06/04492-1
Abstract: 
Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.
Issue Date: 
1-Nov-2009
Citation: 
Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.
Time Duration: 
136-142
Publisher: 
Universidade Federal de Uberlândia (UFU)
Keywords: 
  • Single Nucleotide Polymorphism
  • Interleukin 8
  • PCR-RFLP assay
Source: 
http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
URI: 
http://hdl.handle.net/11449/16026
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/16026
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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