Please use this identifier to cite or link to this item:
- Migratory route of Strongyloides venezuelensis in Lewis rats: Comparison of histological analyses and PCR
- Universidade Estadual Paulista (UNESP)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- Fundação para o Desenvolvimento da UNESP (FUNDUNESP)
- Strongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to characterize the dynamics of S. venezuelensis infection and its migration route in Lewis rats subcutaneously infected with 4000 L3. The dynamics of the infection was determined by counting the number of eggs and by detecting parasite deoxyribonucleic acid in faeces samples. Both techniques similarly detected the infection at day 6 after larvae inoculation. However. PCR performed with the genus primer showed higher sensitivity during the recovery phase. Histological analysis and PCR assay were then used to follow parasite tissue migration. S. venezuelensis migration route included the muscular fibers below the skin, the pulmonary alveoli and the small intestine vilosities. The sensitivity of these two techniques to detect parasite's presence in these tissues was statistically similar. (C) 2010 Elsevier B.V. All rights reserved.
- Experimental Parasitology. San Diego: Academic Press Inc. Elsevier B.V., v. 127, n. 2, p. 334-339, 2011.
- Academic Press Inc. Elsevier B.V.
- Strongyloides venezuelensis
- Migratory route
- Lewis rats
- Histological analysis
- Acesso restrito
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.