Please use this identifier to cite or link to this item:
- Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
- Agência Paulista de Tecnologia dos Agronegócios (APTA)
- Virginia Tech
- Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
- Universidade Estadual Paulista (UNESP)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- Appalachian Farming Systems Research Center (USDA-ARS)
- Virginia Polytechnic Institute
- State University (Virginia Tech)
- USDA-ARS Nematology Lab
- The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.
- Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.
- Elsevier B.V.
- C. elegans
- Anthelmintic plants
- Gastrointestinal nematodes
- Plant extracts
- Acesso aberto
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.