Please use this identifier to cite or link to this item:
- Structures of human purine nucleoside phosphorylase complexed with inosine and ddI
- Universidade Federal do Rio Grande do Sul (UFRGS)
- Universidade Estadual Paulista (UNESP)
- Instituto Butantan
- Pontificia Univ Catolica Rio Grande do Sul
- Human purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effect on B-cell function. PNP is highly specific for 6-oxopurine nucleosides and exhibits negligible activity for 6-aminopurine nucleosides. The catalytic efficiency for inosine is 350,000-fold greater than for adenosine. Adenine nucleosides and nucleotides are deaminated by adenosine deaminase and AMP deaminase to their corresponding inosine derivatives which, in turn, may be further degraded. Here we report the crystal structures of human PNP in complex with inosine and 2',3'-dideoxymosine, refined to 2.8 Angstrom resolution using synchrotron radiation. The present structures provide explanation for ligand binding, refine the purine-binding site, and can be used for future inhibitor design. (C) 2003 Elsevier B.V. All rights reserved.
- Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 313, n. 4, p. 907-914, 2004.
- Elsevier B.V.
- synchrotron radiation
- drug design
- Acesso restrito
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.