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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/20036
Título: 
Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach
Autor(es): 
Instituição: 
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal do Rio Grande do Norte (UFRN)
  • Netherlands Red Cross
  • Indoor Biotechnol Inc
ISSN: 
0091-6749
Resumo: 
Background: Evidence indicates that infection with Ascaris lumbricoides may promote development of allergy and asthma.Objective: To study the role of tropomyosin, a pan-allergen in invertebrates, in IgE responses to A lumbricoides.Methods: Recombinant A lumbricoides and Periplaneta americana tropomyosins were expressed in Pichia pastoris. Levels of IgE to tropomyosins from A lumbricoides and P americana were determined by chimeric ELISA in sera from 119 children living in a parasite-endemic area and 112 patients with cockroach allergy from the allergy clinics. Presence of tropomyosin in A lumbricoides larvae at L3 stage was evaluated by immunofluorescence using mAb IA6, directed against mite tropomyosin. Molecular modeling of P americana and A lumbricoides tropomyosins was performed by using the MODELLER program.Results: A lumbricoides tropomyosin showed 69% to 98% sequence identity to tropomyosins from other invertebrates. The predicted structure of A lumbricoides tropomyosin was similar to that of P americana tropomyosin and showed the characteristic coiled-coil structure. Strong correlation was found for IgE antibodies to tropomyosins from A lumbricoides and P americana in sera from children living in a parasite-endemic area and from patients with cockroach allergy. Larvae of A lumbricoides reacted strongly with mAb IA6.Conclusion: Tropomyosin induces IgE responses in A lumbricoides-infected children and in patients allergic to cockroach.
Data de publicação: 
1-Abr-2008
Citação: 
Journal of Allergy and Clinical Immunology. New York: Mosby-elsevier, v. 121, n. 4, p. 1040-1046, 2008.
Duração: 
1040-1046
Publicador: 
Mosby-elsevier
Palavras-chaves: 
  • tropomyosin
  • Ascaris lumbricoides
  • periplaneta americana
  • IgE cross-reactivity
  • allergy
  • parasites
  • asthma
Fonte: 
http://dx.doi.org/10.1016/j.jaci.2007.12.1147
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/20036
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