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- Effect of C/N Ratio and Physicochemical Conditions on the production of Rhamnolipids by Pseudomonas Aeruginosa LBI
- Universidade Estadual Paulista (UNESP)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- Waste utilization for biosurfactant production is an important approach contributing to the reduction of waste treatment costs, while increasing the economic value of residues and reducing biosurfactant production costs: In an attempt to optimize rhamnolipid production by Pseudomonas aeruginosa LBI, the effects of the oil refinery waste as carbon source, C/N ratio, agitation speed, aeration rate, pH control and fed-batch culture conditions on the production of this biosurfactant were assessed in a bioreactor. Maximum production (16.9 g L(-1)) was attained at a C/N ratio of 23; agitation at 800 rpm and aeration at 2.0 vvm. The rhamnolipid solution (0.1%, w/v) exhibited surface and interface tensions of 25 mN/m and 0.63 mN/m respectively. The critical micelle concentration (CMC) was 73.9 mg L(-1). The optimal parameters used in this study promoted the formation of a useful biosurfactant with potential for many industrial applications.
- Research Journal of Biotechnology. Indore: Research Journal Biotechnology, v. 5, n. 3, p. 19-24, 2010.
- Research Journal Biotechnology
- C/N ratio
- Aeration rate and Agitation speed
- Acesso restrito
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