Você está no menu de acessibilidade

Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/2514
Título: 
Effects of Hormonal Supplementation on Nuclear Maturation and Cortical Granules Distribution of Canine Oocytes During Various Reproductive Stages
Autor(es): 
Instituição: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal do Tocantins (UFT)
  • UNIME Uniao Metropolitana Desenvolvimento Ensino
ISSN: 
0936-6768
Financiador: 
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento: 
  • FAPESP: 03/11319-6
  • FAPESP: 04/07393-4
Resumo: 
The aim of this study was to evaluate the effects of hCG, progesterone and oestradiol supplementation on nuclear and cytoplasmic maturation of canine oocytes cultured for 24, 48, 72 and 96 h. Oocytes obtained from 18 healthy bitches were divided into three groups according to their reproductive status (follicular, luteal and anoestrus stages) and cultured in TCM 199 + 25 UI/ml of hCG + 1 mu g/ml of progesterone + 1 mu g/ml of 17-beta oestradiol or without hormonal supplementation (control) for different periods. Then, they were stained with FITC-LCA-Hoescht for chromatin configuration and cortical granules distribution and evaluated under an epifluorescence microscope. Culture time and the influence of different stages of the oestrous cycle were also evaluated. The present study demonstrated that there was no significant difference among the reproductive stages. With regards to culture medium, only oocytes from the supplemented medium were able to complete meiosis; however, significant difference was only noticed in the percentage of MI stage oocytes (p < 0.05) in the follicular and luteal group at 72 h of culture. Most oocytes in germinal vesicle, germinal vesicle breakdown and metaphase I stage had cortical granules distributed throughout the cytoplasm (immature pattern), irrespective of the culture period (p < 0.05). Cortical granules distributed immediately beneath the plasma membrane (mature) was only observed in metaphase II stage oocytes, but not all of them presented matured cytoplasm. Our results reveal that cortical granules distribution in canine oocytes matured in vitro did not progressed in correspondence with nuclear stage changes and are in accordance with those from other species.
Data de publicação: 
1-Out-2011
Citação: 
Reproduction In Domestic Animals. Malden: Wiley-blackwell, v. 46, n. 5, p. 896-903, 2011.
Duração: 
896-903
Publicador: 
Wiley-Blackwell
Fonte: 
http://dx.doi.org/10.1111/j.1439-0531.2011.01761.x
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/2514
Aparece nas coleções:Artigos, TCCs, Teses e Dissertações da Unesp

Não há nenhum arquivo associado com este item.
 

Itens do Acervo digital da UNESP são protegidos por direitos autorais reservados a menos que seja expresso o contrário.