You are in the accessibility menu

Please use this identifier to cite or link to this item:
Adenosine deaminase activity as a biochemical marker of inflammatory response in goats infected by caprine arthritis-encephalitis virus
  • Universidade Federal Rural da Amazônia (UFRA)
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal do Ceará (UFC)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Caprine arthritis-encephalitis virus (CAEV) is a lentivirus that causes a chronic and degenerative arthritis and is associated with important economic losses. Adenosine deaminase (ADA) levels increase in some human infectious diseases, such as tuberculosis and acquired immune deficiency syndrome. Due to the similarity between CAEV and human immunodeficiency virus, we hypothesized that ADA activity in goats with clinical signs of CAEV infection is also altered and could serve as a valuable biochemical marker. Adenosine deaminase was assayed using adenosine (Ado) or 2'-deoxyadenosine (dAdo) as substrates, and ADA activity was calculated using the amount of ammonium produced. No significant difference was detected in the activity of the serum enzyme when assayed with Ado (K-m = 49.19 +/- 5.28 mu mol/L) or dAdo (K-m = 41.28 +/- 4.58 mu mol/L). Caprine serum ADA is a thermo-stable enzyme and can be stored at cool temperatures for at least 30 days with no loss of activity. An increase in ADA activity (approximately 2.6-fold) was found in serum and synovial fluid in CAEV infected animals with clinical signs of arthritis compared with uninfected animals. In serum, the ADA cutoff value for CAEV disease using Ado was >34.9 U/L. Adenosine deaminase activity may be used as an important biochemical marker of the inflammatory response induced by CAEV, and its determination in serum using adenosine as a simple and inexpensive method is sufficient to assess the pathological condition of the animal. (C) 2012 Elsevier B.V. All rights reserved.
Issue Date: 
Small Ruminant Research. Amsterdam: Elsevier B.V., v. 108, n. 1-3, p. 120-126, 2012.
Time Duration: 
Elsevier B.V.
  • Adenosine deaminase
  • Caprine arthritis-encephalitis
  • ADA cutoff for CAEV disease
Access Rights: 
Acesso restrito
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.