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- Electrochemical and spectroscopic characterization of screen-printed gold-based electrodes modified with self-assembled monolayers and Tc85 protein
- Universidade Estadual Paulista (UNESP)
- Universidade de São Paulo (USP)
- Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- Fundação para o Desenvolvimento da UNESP (FUNDUNESP)
- FAPESP: 05/50574-4
- CNPq: 150670/2007-9
- CNPq: 300728/2007-7
- FUNDUNESP: 01093/05-DFP
- This work investigates the formation of self-assembled monolayers (SAMs) of cystamine and cystamine-glutaraldehyde on a screen-printed electrode, and the immobilization of the Tc85 protein (from Trypanosoma cruzi) on these monolayers. The methods used included infrared techniques, cyclic voltammetry, and electrochemical impedance spectroscopy. The electrochemical studies were performed at pH 6.9 in 0.1 mol L(-1) phosphate buffer solution containing Fe(CN)(6)(-3/-4) redox species. The surface coverage (0) of the electrode was 0.10 (cystamine), 0.35 (cystamine-glutaraldehyde) and 0.84 (Tc85). Interpretation of electrochemical impedance spectroscopy results was based on a charge-transfer reaction involving Fe(CN)(6)(-3/-4) species at high frequencies, followed by a diffusion through the monolayers at lower frequencies. Estimates of the electrode surface coverage, active site radius, and distance between two adjacent sites assumed that charge transfer occurred at the active sites, and that there was a planar diffusion of redox species to these sites. (C) 2009 Elsevier B.V. All rights reserved.
- Journal of Electroanalytical Chemistry. Lausanne: Elsevier B.V. Sa, v. 634, n. 2, p. 111-122, 2009.
- Elsevier B.V. Sa
- Screen-printed electrodes
- Cyclic voltammetry
- Infrared spectroscopy
- Tc85 protein
- Acesso restrito
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