Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/3926
- Title:
- The zymogen-enteropeptidase system: A practical approach to study the regulation of enzyme activity by proteolytic cleavage
- Universidade Estadual Paulista (UNESP)
- 1470-8175
- The present research describes an efficient procedure to obtain high levels of trypsinogen and chymotrypsinogen by using a simple, rapid, and easily reproducible method. The extraction process and the time-course of activation of zymogens can be carried out in a single laboratory period, without sophisticated equipment. The main objective was to prepare a laboratory class that would stimulate student interest in enzyme regulation, exploring the fact that the catalytic activity of some enzymes is regulated by different mechanisms. The regulation of proteolytic enzymes requires the synthesis of an inactive zymogen and its being irreversibly switched on by specific proteolytic cleavage.
- 1-Jan-2004
- Biochemistry and Molecular Biology Education. Bethesda: Amer Soc Biochemistry Molecular Biology Inc., v. 32, n. 1, p. 45-48, 2004.
- 45-48
- Amer Soc Biochemistry Molecular Biology Inc
- zymogen
- trypsin
- chymotrypsin
- enteropeptidase
- enzyme regulation
- proteolytic cleavage
- http://dx.doi.org/10.1002/bmb.2004.494032010311
- http://hdl.handle.net/11449/3926
- Acesso aberto
- outro
- http://repositorio.unesp.br/handle/11449/3926
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.