You are in the accessibility menu

Please use this identifier to cite or link to this item:
FISH using a gag-like fragment probe reveals a common Ty3-gypsy-like retrotransposon in genome of Coffea species
  • Universidade Estadual de Londrina (UEL)
  • Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
  • Inst Agronom Parana
  • Universidade Estadual Paulista (UNESP)
  • Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
  • Instituto Agronômico do Paraná (IAPAR)
  • Consorcio Pesquisa Cafe
  • INCT Cafe
The genus Coffea possesses about 100 species, and the most economically important are Coffea canephora and Coffea arabica. The latter is predominantly self-compatible with 2n = 4x = 44, while the others of the genus are diploid with 2n = 2x = 22 and mostly self-incompatible. Studies using molecular markers have been useful to detect differences between genomes in Coffea; however, molecular and cytogenetic studies have produced only limited information on the karyotypes organization. We used DOP-PCR to isolate repetitive elements from genome of Coffea arabica var. typica. The pCa06 clone, containing a fragment of 775 bp length, was characterized by sequencing and used as a probe in chromosomes of C. arabica and six other species: C. canephora, Coffea eugenioides, Coffea kapakata, Coffea liberica var. dewevrei, Coffea racemosa, and Coffea stenophylla. This insert shows similarities with a gag protein of the Ty3-gypsy-like super-family. Dot blot and FISH analyses demonstrated that pCa06 is differentially accumulated between species and chromosomes. Signals appeared scattered and clustered on the chromosomes and were also associated with heterochromatic regions. While the literature shows that there is a high karyotype similarity between Coffea species, our results point out differences in the accumulation and dispersion of this Ty3-gypsy-like retrotransposon during karyotype differentiation of Coffea.
Issue Date: 
Genome. Ottawa: Canadian Science Publishing, Nrc Research Press, v. 55, n. 12, p. 825-833, 2012.
Time Duration: 
Canadian Science Publishing, Nrc Research Press
  • Coffea
  • dot blot
  • Fish
  • gag sequence
  • Retrotransposon
  • Ty3-gypsy-like
Access Rights: 
Acesso restrito
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.