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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/63647
Title: 
Protein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
0022-1422
Abstract: 
To investigate further the age-related reduction in muscle protein synthesis activity found previously using a crude polyribosome/pH 5 system (Pluskal et al., 1984), a 0.5M KCl washing procedure was utilized to remove the nonribosomal factors from polyribosomes isolated from male Sprague-Dawley rats in the following age groups: young (1 to 2 months), mature (12 months), and aged (22 to 24 months). Using a common source of enriched elongation factor fraction from young animals, it was not possible to demonstrate any significant difference (p > .05) in protein synthesis between the 0.5M KCl-washed polyribosomes isolated from the various age groups. Using a cell-free system containing young salt washed polyribosomes stimulated by the addition of 0.5M KCl-wash fractions, however, it was shown that the mature and aged salt-wash fractions were less (p < .05) active than material from young animals. Thus, the observed decline in protein synthesis efficiency during aging may be attributed to a reduced capacity to promote initiation/elongation by the nonribosomal salt wash fractions of muscle polyribosomes.
Issue Date: 
1-Jan-1984
Citation: 
Journals of Gerontology, v. 39, n. 4, p. 392-397, 1984.
Time Duration: 
392-397
Keywords: 
  • age
  • aging
  • animal experiment
  • cytology
  • muscle
  • nonhuman
  • polysome
  • protein synthesis
  • rat
  • Aging
  • Amino Acids
  • Animal
  • Cell-Free System
  • In Vitro
  • Male
  • Polyribosomes
  • Potassium Chloride
  • Proteins
  • Rats
  • Rats, Inbred Strains
  • Support, Non-U.S. Gov't
  • Support, U.S. Gov't, P.H.S.
Source: 
http://dx.doi.org/10.1093/geronj/39.4.392
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/63647
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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