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- Title:
- Protein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes
- Universidade Estadual Paulista (UNESP)
- 0022-1422
- To investigate further the age-related reduction in muscle protein synthesis activity found previously using a crude polyribosome/pH 5 system (Pluskal et al., 1984), a 0.5M KCl washing procedure was utilized to remove the nonribosomal factors from polyribosomes isolated from male Sprague-Dawley rats in the following age groups: young (1 to 2 months), mature (12 months), and aged (22 to 24 months). Using a common source of enriched elongation factor fraction from young animals, it was not possible to demonstrate any significant difference (p > .05) in protein synthesis between the 0.5M KCl-washed polyribosomes isolated from the various age groups. Using a cell-free system containing young salt washed polyribosomes stimulated by the addition of 0.5M KCl-wash fractions, however, it was shown that the mature and aged salt-wash fractions were less (p < .05) active than material from young animals. Thus, the observed decline in protein synthesis efficiency during aging may be attributed to a reduced capacity to promote initiation/elongation by the nonribosomal salt wash fractions of muscle polyribosomes.
- 1-Jan-1984
- Journals of Gerontology, v. 39, n. 4, p. 392-397, 1984.
- 392-397
- age
- aging
- animal experiment
- cytology
- muscle
- nonhuman
- polysome
- protein synthesis
- rat
- Aging
- Amino Acids
- Animal
- Cell-Free System
- In Vitro
- Male
- Polyribosomes
- Potassium Chloride
- Proteins
- Rats
- Rats, Inbred Strains
- Support, Non-U.S. Gov't
- Support, U.S. Gov't, P.H.S.
- http://dx.doi.org/10.1093/geronj/39.4.392
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/63647
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