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The effect of serum on in vitro maturation, in vitro fertilization and steroidogenesis of bovine oocytes co-cultured with granulosa cells.
Universidade Estadual Paulista (UNESP)
The influence of fetal calf serum alone (FCS) or associated with proestrous (FCS+PCS), estrous (FCS+ECS) or metaestrous (FCS+MCS) cow serum added to the culture medium and of the steroids produced by co-cultured granulosa cells were evaluated in terms of the in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes. Supplementation of the medium with FCS+ECS and FCS+MCS resulted in higher proportions of oocytes that reached metaphase II (96.0% and 93.3%, respectively) and in higher proportions of embryos that reached the four- and eight-cell/morula stages (51.9% and 65.6%, respectively), whereas the supplementation with FCS and FCS+PCS resulted in only 79.2% and 67.5%, respectively, of matured oocytes and 26.7% and 34.3%, respectively, of cleaved embryos. These findings show that the best IVM and IVF were obtained at lower concentrations of estradiol produced by co-cultured granulosa cells (supplementation with FCS+ECS: 10.3 ng/ml and FCS+MCS: 2.1 ng/ml), whereas the worst results in IVM and IVF occurred at higher concentrations of estradiol that were obtained with FCS (33.1 ng/ml) and FCS+PCS (19.9 ng/ml) supplementation. These data suggest an inhibitory effect of estradiol on resumption of oocyte meiosis in vitro.
Issue Date: 
Brazilian Journal of Medical and Biological Research, v. 28, n. 2, p. 213-217, 1995.
Time Duration: 
  • steroid
  • animal
  • biosynthesis
  • cattle
  • cell culture
  • culture medium
  • estrus
  • female
  • fertilization in vitro
  • fetus blood
  • granulosa cell
  • growth, development and aging
  • oocyte
  • physiology
  • prenatal development
  • Animal
  • Cattle
  • Cells, Cultured
  • Culture Media
  • Embryo and Fetal Development
  • Estrus
  • Female
  • Fertilization in Vitro
  • Fetal Blood
  • Granulosa Cells
  • Oocytes
  • Steroids
  • Support, Non-U.S. Gov't
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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