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Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
  • Universidade Estadual Paulista (UNESP)
  • Universidade Estadual de Campinas (UNICAMP)
We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ± 0.06% and 2.08 ± 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ± 0.15% and 3.09 ± 0.21 % of the islet insulin content in control and 2.43 ± 0.16% and 4.31 ± 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 μg/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.
Issue Date: 
Brazilian Journal of Medical and Biological Research, v. 30, n. 3, p. 359-361, 1997.
Time Duration: 
  • Glucose
  • Glucose transporter
  • Pancreatic islets
  • Prolactin
  • glucose
  • glucose transporter
  • glucose transporter 2
  • prolactin
  • animal
  • biosynthesis
  • culture technique
  • metabolism
  • pancreas islet
  • physiology
  • rat
  • Animals
  • Cell Culture Techniques
  • Glucose Transporter Type 2
  • Islets of Langerhans
  • Monosaccharide Transport Proteins
  • Rats
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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