You are in the accessibility menu

Please use this identifier to cite or link to this item:
An enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against babesia boris in cattle
  • Universidade Estadual Paulista (UNESP)
  • Inst. Biologico de Sao Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
A method for the isolation of Babesia bovis merozoites from infected erythrocytes (Machado et al., 1994) and an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-B. bovis antibodies were developed. This ELISA utilizes a soluble, alkali-digested B. bovis antigen. Sera from calves experimentally infected with B. bovis were screened by this technique from day 9 to day 233 postinfection (PI). Maximum tilers were reached between days 29 and 149 PI. Sera from calves (n =62), heifers (n =38) and cows (n = 49), raised in tick-infested areas of Sao Paulo State, showed higher antibody levels in heifers and cows. A higher percentage of negative sera (19.4%) was found among calves. Sodium dodecyl sulphate-polyacrylamide electrophoresis (SDS-PAGE) and immunoblotting have identified proteins of similar molecular mass in the two species. Sera from calves experimentally infected with B. bovis reacted with homologous antigens at the level of 95, 66 and 23 kDa. The same serum reacted with the 23 kDa band of heterologous antigen. Sera front calves experimentally infected with B. bigemina recognized 82, 66, 58, 36 and the 23 kDa polypeptides of homologous and heterologous antigens. The experimental ELISA described may prove to he a practical serological test for bovine Babesia infection with the choice of specific test antigen for B. bovis and B. bigemina.
Issue Date: 
Veterinary Parasitology, v. 71, n. 1, p. 17-26, 1997.
Time Duration: 
  • Babesia bigemina
  • Babesia hovis
  • Cattle
  • Western blotting
  • animal cell
  • animal parasitosis
  • animal tissue
  • antibody detection
  • antibody titer
  • babesia
  • cattle
  • enzyme linked immunosorbent assay
  • immunoblotting
  • merozoite
  • nonhuman
  • parasite isolation
  • serodiagnosis
  • Animals
  • Antibodies, Protozoan
  • Antibody Formation
  • Babesia bovis
  • Babesiosis
  • Blotting, Western
  • Cattle Diseases
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Time Factors
  • Acari
  • Animalia
  • Babesia
  • Bos taurus
  • Bovinae
Access Rights: 
Acesso restrito
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.