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Chromosome damage induced by 5-azacytidine under the influence of caffeine or cytosine arabinoside in CHO-K1 (wild-type) and XRS-5 (mutant) cell lines
  • Universidade de São Paulo (USP)
  • Universidade Estadual Paulista (UNESP)
5-azacytidine (5-azaC) treatment combined with cytosine arabinoside (ara-C) or caffeine were performed in vitro in Chinese hamster cells, CHO-K1 (wild-type) and xrs-5 (mutant) cell lines, in order to compare the cell response to the induction of chromosomal aberrations. Exponentially growing cells were treated with 5-azaC (4-16 uM) for 1 h, the cells were washed and incubated for 7 h, and 500 uM caffeine or 5 uM ara-C were added to the cultures for the last 2 h. In both cell lines, 5-azaC induced a significantly increase (P<0.01) in the frequencies of aberrations; in the combined treatments (5-azaC + Ara-C), a significant reduction (P<0.05) was observed for the aberrations which were randomly distributed. Caffeine had no influence at the same conditions. 5-azaC induced-DNA lesions were probably processed at S/G2 phase in a common pathway in both cell lines, but alternatively, 5-azaC may cause xrs-5 cells to revert to the wild-type.
Issue Date: 
Cancer Research, Therapy and Control, v. 11, n. 2, p. 125-134, 2001.
Time Duration: 
  • 5-Azacytidine
  • Caffeine
  • Chromosomal aberrations
  • Cytosine arabionside
  • azacitidine
  • caffeine
  • cytarabine
  • animal cell
  • cell cycle G2 phase
  • cell cycle S phase
  • cell growth
  • cell line
  • CHO cell
  • chromosome aberration
  • chromosome damage
  • controlled study
  • nonhuman
  • priority journal
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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