Papilomavírus humano (HPV) em lesões benignas e malignas de mucosa oral pelos métodos de imuno-histoquímica e hibridização in situ

Abstract

Fifteen oral biopsies with histopathological diagnosis of oral papilloma (n=7) and invasive squamous cell carcinoma (n=8) have been investigated for the presence of human papillomavirus (HPV) by immunohistochemistry (IHC) and in situ hybridization (ISH). HPV capsid proteins were evaluated by IHC (immunohistochemistry) with polyclonal antibody and the viral DNA analyzed by in situ hybridization with wide-spectrum, 6/11 and 16/18 biotinilated probes. HPV was detected by IHC in 13% (n=2) and, by ISH, in 60% (n=9) of the oral lesions. The two biopsies which immunostaned positive for HPV also exhibited HPV DNA 6/11. Moreover, HPV 16/18 was found in 20% of the oral carcinomas and 7% of the oral papillomas, while HPV 6/11 was detected in 7% of the carcinomas and 26% of the papillomas. It can be concluded that IHC is low efficient for the diagnosis of oral HPV and the presence of HPV 16/18 in benign and malignant lesions sugests the participation of this virus in oral carcinogenesis.