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- Botryosphaeran, a new substrate for the production of beta-1,3-glucanases by Botryosphaeria rhodina and Trichoderma harzianum Rifai
- Universidade Estadual de Londrina (UEL)
- Universidade Estadual Paulista (UNESP)
- Botryosphaeria rhodina and Trichoderma harzianum Rifai were grown on botryosphaeran (an exopolysaccharide (EPS) of the beta-1,3; 1,6-D-Glucan type produced by B. rhodina) as sole carbon source with the objective of producing beta-glucanases of the beta-type. Conditions for beta-1,3-glucanase production by T harzianum were examined by a statistical response surface method, and showed maximal enzyme production at 5 days growth in media containing 1.5 g/1 of EPS. Good agreement was obtained between the experimental values of beta-1, 3-glucanase activity and the corresponding values predicted by the mathernatical model. The crude beta-1,3-glucanase preparations were active towards a number of different beta-1,3-glucans and beta-glucosides. The mycelium of B. rhodina also proved to be a good substrate for beta-1,3-glucanase production by both fungal species. (c) 2005 Published by Elsevier Ltd.
- Process Biochemistry. Oxford: Elsevier B.V., v. 40, n. 12, p. 3783-3788, 2005.
- Elsevier B.V.
- Botryosphaeria rhodina
- exopolysaccharide beta-1,3-glucanases
- Trichoderma harzianus Rifai
- response surface methodology
- Acesso restrito
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