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Effectiveness of microwave irradiation on the disinfection of complete dentures
Universidade Estadual Paulista (UNESP)
Purpose: The purpose of this study was to evaluate the effectiveness of microwave irradiation on the disinfection of simulated complete dentures. Materials and Methods: Eighty dentures were fabricated in a standardized procedure and subjected to ethylene oxide sterilization. The dentures were individually inoculated (10 7 cfu/mL) with tryptic soy broth (TSB) media containing one of the tested microorganisms (Candida albicans, Streptoccus aureus, Bacillus subtilis, and Pseudomonas aeruginosa). After 48 hours of incubation at 37°C, 40 dentures were individually immersed in 200 mL of water and submitted to microwave irradiation at 650 W for 6 minutes. Forty nonirradiated dentures were used as positive controls. Replicate aliquots (25 μL) of suspensions were plated at dilutions of 10 -3 to 10 -6 on plates of selective media appropriate for each organism. All plates were incubated at 37°C for 48 hours. TSB beakers with the microwaved dentures were incubated at 37°C for 7 more days. After incubation, the number of colony-forming units was counted and the data were statistically analyzed by Kruskal-Wallis test (α = .05). Results: No evidence of growth was observed at 48 hours for S aureus, B subtilis, and C albicans. Dentures contaminated with P aeruginosa showed small growth on 2 plates. After 7 days incubation at 37°C, no growth was visible in the TSB beakers of S aureus and C albicans. Turbidity was observed in 3 broth beakers, 2 from P aeruginosa and 1 from B subtilis. Conclusion: Microwave irradiation for 6 minutes at 650 W produced sterilization of complete dentures contaminated with S aureus and C albicans and disinfection of those contaminated with P aeruginosa and B subtilis.
Issue Date: 
International Journal of Prosthodontics, v. 19, n. 3, p. 288-293, 2006.
Time Duration: 
  • acrylic acid resin
  • disinfectant agent
  • ethylene oxide
  • Lucitone
  • poly(methyl methacrylate)
  • Bacillus subtilis
  • bacterial count
  • Candida albicans
  • culture medium
  • denture
  • disinfection
  • instrument sterilization
  • materials testing
  • microwave radiation
  • Pseudomonas aeruginosa
  • radiation exposure
  • Staphylococcus aureus
  • Acrylic Resins
  • Colony Count, Microbial
  • Culture Media
  • Denture Bases
  • Denture, Complete
  • Ethylene Oxide
  • Materials Testing
  • Microwaves
  • Polymethyl Methacrylate
  • Sterilization
  • Time Factors
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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