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Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: Insights on the origin of protoluciferase activity and bioluminescence colours
  • Universidade Estadual Paulista (UNESP)
  • Universidade Federal de São Carlos (UFSCar)
  • Institute of Advanced Industrial Science and Technology (AIST)
  • 1522-7235
  • 1522-7243
Luciferyl adenylate, the key intermediate in beetle bioluminescence, is produced through adenylation of D-luciferin by beetle luciferases and also by mealworm luciferase-like enzymes which produce a weak red chemiluminescence. However, luciferyl adenylate is only weakly chemiluminescent in water at physiological pH and it is unclear how efficient bioluminescence evolved from its weak chemiluminescent properties. We found that bovine serum albumin (BSA) and neutral detergents enhance luciferyl adenylate chemiluminescence by three orders of magnitude, simulating the mealworm luciferase-like enzyme chemiluminescence properties. These results suggest that the beetle protoluciferase activity arose as an enhanced luciferyl adenylate chemiluminescence in the protein environment of the ancestral AMP-ligase. The predominance of luciferyl adenylate chemiluminescence in the red region under most conditions suggests that red luminescence is a more primitive condition that characterized the original stages of protobioluminescence, whereas yellow-green bioluminescence may have evolved later through the development of a more structured and hydrophobic active site. Copyright © 2006 John Wiley & Sons, Ltd.
Issue Date: 
Luminescence, v. 21, n. 4, p. 262-267, 2006.
Time Duration: 
  • AMP ligases
  • Bioluminescence
  • Chemiluminescence
  • Luciferase
  • Luciferyl adenylate
  • Mealworm
  • Protobioluminescence
  • Tenebrio
  • adenosine phosphate
  • adenosine triphosphate
  • bovine serum albumin
  • detergent
  • luciferase
  • luciferin
  • water
  • animal
  • beetle
  • cattle
  • chemistry
  • chemoluminescence
  • enzymology
  • metabolism
  • methodology
  • pH
  • sensitivity and specificity
  • solution and solubility
  • Adenosine Monophosphate
  • Adenosine Triphosphate
  • Animals
  • Beetles
  • Cattle
  • Chemiluminescent Measurements
  • Detergents
  • Firefly Luciferin
  • Hydrogen-Ion Concentration
  • Luciferases
  • Sensitivity and Specificity
  • Serum Albumin, Bovine
  • Solutions
  • Water
  • Bos taurus
  • Coleoptera
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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