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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/69102
Título: 
Determinação precoce do sexo fetal pela análise do DNA no plasma materno
Título alternativo: 
Early determination of gender by analysis of fetal DNA in maternal plasma
Autor(es): 
Instituição: 
  • Centro Paulista de Diagnóstico e Pesquisa - CPDP
  • Universidade Estadual Paulista (UNESP)
  • RDO Diagnósticos Médicos
  • Centro Paulista de Diagnóstico e Pesquisa
  • Centro de Reprodução Humana - Prof. Franco Junior
ISSN: 
0100-7203
Resumo: 
Purpose: to verify the viability of early diagnosis of fetal gender in maternal plasma by the real-time polymerase chain reaction (real-time PCR) starting at the 5th week of pregnancy. Methods: peripheral blood was collected from pregnant women with single fetus starting at the 5th week of gestation. After centrifugation, 0.4 mL plasma was separated for fetal DNA extraction. The DNA was analyzed in duplicate by real-time PCR for two genomic regions, one of the Y chromosome and the other common to both sexes, through the TaqMan® method, which uses a pair of primers and a fluorescent probe. Patients who aborted were excluded. Results: a total of 79 determinations of fetal DNA in maternal plasma were performed in 52 pregnant women. The results of the determinations were compared to fetal gender after delivery. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks with 87% sensitivity, and 95.6% (22 of 23 cases) at 6 weeks with 92% sensitivity. Starting at the 7th week of pregnancy, accuracy was 100% (29 of 29 cases). Specificity was 100% regardless of gestational age. Conclusion: real-time PCR for the detection of fetal gender in maternal plasma starting at the 5th week of gestation has good sensitivity and excellent specificity. There was agreement of the results in 100% of the cases in which male gender was diagnosed, regardless of gestational age, and from the 7th week of gestation for female gender diagnosis.
Data de publicação: 
27-Set-2006
Citação: 
Revista Brasileira de Ginecologia e Obstetricia, v. 28, n. 3, p. 190-194, 2006.
Duração: 
190-194
Palavras-chaves: 
  • Gestational age
  • Polymerase chain reaction/methods
  • Pre-natal diagnosis
  • Sex determination (Analysis)
Fonte: 
http://dx.doi.org/10.1590/S0100-72032006000300009
Endereço permanente: 
Direitos de acesso: 
Acesso aberto
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/69102
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