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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/6937
Título: 
Up-converter nanophosphor Y2O2S:Er,Yb aminofunctionalized containing or not spherical silica conjugated with BSA
Autor(es): 
Instituição: 
Universidade Estadual Paulista (UNESP)
ISSN: 
0022-2313
Resumo: 
This work reports on the study of the nanophosphor. Y2O2S:Er(2%),Yb(1%) obtained from polymeric resin to be evaluated as fluorescent label with Suitable features to conjugate with bio-molecules for bioassay up-converting phosphor technology (UPT) application A conjugation protocol between bovine serum albumin (BSA) and the aminofunctionalized nanophosphor containing or not spherical silica was established UV-vis results indicated an effective conjugation between nanophosphor particles and the protein up-conversion measurements under 980 nm excitation performed for samples before and after aminofunctionalization showed that nanophosphor particles luminescence features keep unchanged in all cases All results suggest that the adapted protocol is feasible to provide a nanoparticle-protein effective conjugation preserving nanophosphor optical features The presence of spherical silica can be considered advantageous to increase conjugation efficiency Therefore. the developed procedure is applicable for future conjugations between the chosen nanophosphor and the streptavidin protein chat takes part in the well known self-recognition system avidin-biotin. (C) 2009 Elsevier B.V All rights reserved.
Data de publicação: 
1-Dez-2009
Citação: 
Journal of Luminescence. Amsterdam: Elsevier B.V., v. 129, n. 12, p. 1726-1730, 2009.
Duração: 
1726-1730
Publicador: 
Elsevier B.V.
Palavras-chaves: 
  • Functionalised spherical silica
  • Up-converter
  • Nanophosphor
  • Label agent
  • Biological assay
Fonte: 
http://dx.doi.org/10.1016/j.jlumin.2009.04.029
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/6937
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