You are in the accessibility menu

Please use this identifier to cite or link to this item:
Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.
Universidade Estadual Paulista (UNESP)
A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.
Issue Date: 
Journal of oral science, v. 49, n. 3, p. 213-220, 2007.
Time Duration: 
  • chelating agent
  • edetic acid
  • fibroblast growth factor 2
  • protein synthesis inhibitor
  • tetracycline
  • cell adhesion
  • cell culture
  • cell proliferation
  • cell shape
  • cytology
  • dentin
  • drug effect
  • fibroblast
  • human
  • pathology
  • periodontitis
  • regression analysis
  • tooth root
  • Cell Adhesion
  • Cell Proliferation
  • Cell Shape
  • Cells, Cultured
  • Chelating Agents
  • Dentin
  • Edetic Acid
  • Fibroblast Growth Factor 2
  • Fibroblasts
  • Humans
  • Periodontitis
  • Protein Synthesis Inhibitors
  • Regression Analysis
  • Tetracycline
  • Tooth Root
Access Rights: 
Acesso aberto
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.