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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/70097
Title: 
Immunohistochemistry assay to detect Turkey coronavirus (TCoV) from experimentally infected poults
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
1553-619X
Sponsorship: 
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Abstract: 
The objective of this study was to develop a direct immunohistochemical assay to detect TCoV antigens in formalin-fixed paraffin-embedded sections prepared from experimentally infected poults. The sections of ileo, ileo-cecal junction and ceca regions from intestine were prepared and submitted to two different primary antibodies, first the non-biotin labeled polyclonal antibody for the indirect method, and second the biotin-labeled polyclonal antibody, both raised against IBV by immunized specific pathogen free chickens. All sections were submitted to immufluorescent assay (IFA), a conventional method, and the results compared. The direct immunohistochemical technique showed a higher frequency of antigen in tissues, especially from the ileo-cecal junction with no difference between results obtained by the conventional method. Finally, the immunofluorescence and all modalities of molecular approaches have been played an important role to the diagnosis and prevention of TCoV infections, although to be precise on infectious disease diagnosis, it is necessary complementary techniques. Here, was standardized the biotin labeled polyclonal antibody as reliable tool to be used as an alternative detection of Turkey Coronavirus. © 2007 Science Publications.
Issue Date: 
1-Dec-2007
Citation: 
American Journal of Immunology, v. 3, n. 2, p. 31-34, 2007.
Time Duration: 
31-34
Keywords: 
  • IFA
  • Immunohistochemistry
  • PEMS
  • TCoV
  • biotin
  • polyclonal antibody
  • animal experiment
  • animal model
  • animal tissue
  • antibody labeling
  • antigen detection
  • Avian infectious bronchitis virus
  • bird disease
  • controlled study
  • Coronavirus
  • embryo
  • histopathology
  • immunization
  • immunofluorescence
  • immunohistochemistry
  • intermethod comparison
  • intestine infection
  • nonhuman
  • turkey (bird)
  • virus detection
  • virus infection
Source: 
http://dx.doi.org/10.3844/ajisp.2007.31.34
URI: 
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/70097
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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