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Cytotoxic effects of different concentrations of chlorhexidine
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
Purpose: To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. Methods: The odontoblast-like cells were seeded (30,000 cells/cm 2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H 2O 2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. Results: The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On theother hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.
Issue Date: 
American Journal of Dentistry, v. 20, n. 6, p. 400-404, 2007.
Time Duration: 
  • buffer
  • chlorhexidine
  • coloring agent
  • diagnostic agent
  • hydrogen peroxide
  • oxidizing agent
  • sodium chloride
  • tetrazolium
  • thiazole derivative
  • thiazolyl blue
  • topical antiinfective agent
  • unclassified drug
  • animal
  • cell adhesion
  • cell line
  • cell membrane
  • cell shape
  • comparative study
  • dose response
  • drug effect
  • metabolism
  • mouse
  • odontoblast
  • pathology
  • ultrastructure
  • Animals
  • Anti-Infective Agents, Local
  • Buffers
  • Cell Adhesion
  • Cell Line
  • Cell Membrane
  • Cell Shape
  • Chlorhexidine
  • Coloring Agents
  • Dose-Response Relationship, Drug
  • Hydrogen Peroxide
  • Mice
  • Odontoblasts
  • Oxidants
  • Sodium Chloride
  • Tetrazolium Salts
  • Thiazoles
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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