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Results of the GEP-ISFG collaborative study on an X-STR Decaplex
  • IPATIMUP (Institute of Pathology and Molecular Immunology of University of Porto)
  • Universidade de Santiago de Compostela
  • Unidad de Medicina Legal
  • Banco Nacional de Datos Genéticos
  • Biologia Molecular Ltda (BIOMOL Ltda)
  • Universidad Complutense de Madrid
  • Facultad de Farmacia y Bioquímica
  • Instituto Nacional de Medicina Legal
  • Dirección General de la Guardia Civil
  • la Universidad
  • Universidade do Estado do Rio de Janeiro (UERJ)
  • Universidade Estadual Paulista (UNESP)
  • Delegação de Lisboa. Instituto Nacional de Medicina Legal
  • Servicio Genómica
  • Area de Laboratorio Ertzaintza
  • ADF TecnoGen S.L.
  • Delegación de Canarias
  • Setor de Biologia Molecular
  • Agencia Córdoba Ciencia
  • Laboratorio de Biología
  • Facultad de Medicina
  • PRICAI-Fundación Favaloro
  • GDPN-Genetica Medica e Diagnostico Pre-Natal Prof. Doutor Sergio Castedo, S.A. Porto
  • Poder Judicial
  • Policlínica Gipuzkoa
  • Laboratorio de Inmunogenética y Diagnóstico Molecular (LIDMO)
  • Universitat Pompeu Fabra
A collaborative work was carried out by the Spanish and Portuguese ISFG Working Group with a PCR multiplex for X chromosome STRs. Markers were selected among those described as polymorphic in humans and that have been used by some laboratories in forensics. Primers and various technical methods were investigated with the aim of optimizing a multiplex for the 10 selected X-STRs. Primer mix stock solutions were sent to the laboratories that were asked to analyse two female bloodstains, taking as reference the genetic profiles from 9947A, 9948 and NA3657 samples. In this work, we report the results obtained by 30 GEP-ISFG laboratories, using this Decaplex, as well as alternative technical conditions that also produced good results. © 2008 Elsevier B.V. All rights reserved.
Issue Date: 
Forensic Science International: Genetics Supplement Series, v. 1, n. 1, p. 677-679, 2008.
Time Duration: 
  • Multiplex PCR
  • X-STRs
  • controlled study
  • electrophoresis
  • female
  • forensic genetics
  • genetic marker
  • human
  • laboratory test
  • polymerase chain reaction
  • priority journal
  • reference value
  • short tandem repeat
  • silver staining
  • X chromosome
Access Rights: 
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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