Please use this identifier to cite or link to this item:
http://acervodigital.unesp.br/handle/11449/70966
- Title:
- The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially Phosphorylates dCMP
- Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
- Universidade Estadual Paulista (UNESP)
- 0021-9193
- The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
- 1-Apr-2009
- Journal of Bacteriology, v. 191, n. 8, p. 2884-2887, 2009.
- 2884-2887
- cytidine phosphate kinase
- deoxycytidine phosphate
- monomer
- phosphotransferase
- unclassified drug
- cytidylate kinase
- nucleoside monophosphate kinase
- recombinant protein
- bacterial gene
- bacterial strain
- controlled study
- enzyme kinetics
- enzyme phosphorylation
- gene expression
- gene locus
- genetic code
- molecular cloning
- Mycobacterium tuberculosis
- nonhuman
- priority journal
- protein function
- protein purification
- steady state
- amino acid sequence
- enzymology
- genetics
- isolation and purification
- kinetics
- metabolism
- molecular genetics
- phosphorylation
- sequence alignment
- Amino Acid Sequence
- Cloning, Molecular
- Deoxycytidine Monophosphate
- Gene Expression
- Kinetics
- Molecular Sequence Data
- Nucleoside-Phosphate Kinase
- Phosphorylation
- Recombinant Proteins
- Sequence Alignment
- http://dx.doi.org/10.1128/JB.01337-08
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/70966
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.