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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/73519
Title: 
Determinação de GMP e CMP* no leite por métodos espectrofotométrico (ANSM) e cromatográfico (HPLC) - parâmetros metodológicos (*glicomacropeptídeo e caseinomacropeptídeo)
Other Titles: 
Determination of GMP and CMP in milk by spectrophotometric - ANSM, and by chromatographic - HPLC, methods - methodological parameters
Author(s): 
Institution: 
Universidade Estadual Paulista (UNESP)
ISSN: 
1517-784X
Abstract: 
Glycomacropeptide is a glycosilated fraction of bovine kappa-casein that remains soluble when milk is clotted by rennin. Determinations of milk sialic acid content are useful because its concentration reflects the amount of free GMP of milk. In normal milk these amounts are very low, 12 to 16 times lower than in sweet whey. Therefore, its determination may be applied to verify possible frauds with whey addictions, since it works as a fingerprint. With the description of a new spectrophotometric method for determination of free GMP (ANSM) occurred a simplification of procedures, being faster than others (HPLC method), without loss of accuracy. However, due to variations of glycosilation in kappa-casein between animals, during the lactation period, due to mastitis and yet due to proteolysis on milk, it was necessary to know these variations to interpret correctly the analytical results. It was analyzed 1,703 samples of producer's raw milk and 1,189 samples of processed milk (HTST and UHT). The results showed that normal milk from herd (producer's milk) have only small amounts of free GMP, with A470nm = 0.232±0.088 or 3.89±1.25 mg of sialic acid/L. The upper limit of this distribution was A = 0.496; thus every bigger value may represent a problem, being outside of normal distribution.
Issue Date: 
30-Aug-2012
Citation: 
Archives of Veterinary Science, v. 17, n. 2, p. 29-39, 2012.
Time Duration: 
29-39
Keywords: 
  • Analysis
  • Fraud
  • Interpretation
  • Milk proteolysis
  • Sweet whey
Source: 
http://ojs.c3sl.ufpr.br/ojs/index.php/veterinary/article/view/16554
URI: 
Access Rights: 
Acesso aberto
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/73519
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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