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Utilize este identificador para citar ou criar um link para este item: http://acervodigital.unesp.br/handle/11449/74169
Título: 
A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells
Autor(es): 
Instituição: 
  • University of Michigan
  • Universidade Estadual Paulista (UNESP)
  • University of the Pacific
ISSN: 
0109-5641
Resumo: 
Objectives: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. Methods: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Significance: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. © 2012 Academy of Dental Materials.
Data de publicação: 
1-Jan-2013
Citação: 
Dental Materials, v. 29, n. 1, p. 97-102, 2013.
Duração: 
97-102
Palavras-chaves: 
  • Dental pulp
  • Hydrogel
  • Odontoblast
  • Regenerative Endodontics
  • Stem cells
  • Tissue engineering
  • 3-d modeling
  • Cell morphology
  • Cell viability
  • Cell-based
  • Clinical translation
  • Human teeths
  • Hydrogel scaffolds
  • Injectable scaffolds
  • Morphological features
  • Physiological condition
  • PuraMatrix
  • Self-assembling peptides
  • Cell culture
  • Confocal microscopy
  • Dentistry
  • Hydrogels
  • Scaffolds (biology)
Fonte: 
http://dx.doi.org/10.1016/j.dental.2012.08.002
Endereço permanente: 
Direitos de acesso: 
Acesso restrito
Tipo: 
outro
Fonte completa:
http://repositorio.unesp.br/handle/11449/74169
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