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http://acervodigital.unesp.br/handle/11449/75268
- Title:
- Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production
- Universidade Estadual Paulista (UNESP)
- Universidade de São Paulo (USP)
- 1067-1927
- 1524-475X
- The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C - Control (nontreated), A - Artin M gel, and V - Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. © 2013 by the Wound Healing Society.
- 1-May-2013
- Wound Repair and Regeneration, v. 21, n. 3, p. 456-463, 2013.
- 456-463
- collagen fiber
- interleukin 1
- interleukin 6
- lectin
- transforming growth factor beta
- vasculotropin
- animal cell
- animal tissue
- cell proliferation
- cheek mucosa
- collagen synthesis
- controlled study
- enzyme linked immunosorbent assay
- epithelization
- fibroblast culture
- gel
- histology
- immunohistochemistry
- in vivo study
- male
- mouth lesion
- nonhuman
- priority journal
- protein expression
- rat
- secretion (process)
- surgical wound
- wound healing
- http://dx.doi.org/10.1111/wrr.12041
- Acesso restrito
- outro
- http://repositorio.unesp.br/handle/11449/75268
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