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Please use this identifier to cite or link to this item: http://acervodigital.unesp.br/handle/11449/75268
Title: 
Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production
Author(s): 
Institution: 
  • Universidade Estadual Paulista (UNESP)
  • Universidade de São Paulo (USP)
ISSN: 
  • 1067-1927
  • 1524-475X
Abstract: 
The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C - Control (nontreated), A - Artin M gel, and V - Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. © 2013 by the Wound Healing Society.
Issue Date: 
1-May-2013
Citation: 
Wound Repair and Regeneration, v. 21, n. 3, p. 456-463, 2013.
Time Duration: 
456-463
Keywords: 
  • collagen fiber
  • interleukin 1
  • interleukin 6
  • lectin
  • transforming growth factor beta
  • vasculotropin
  • animal cell
  • animal tissue
  • cell proliferation
  • cheek mucosa
  • collagen synthesis
  • controlled study
  • enzyme linked immunosorbent assay
  • epithelization
  • fibroblast culture
  • gel
  • histology
  • immunohistochemistry
  • in vivo study
  • male
  • mouth lesion
  • nonhuman
  • priority journal
  • protein expression
  • rat
  • secretion (process)
  • surgical wound
  • wound healing
Source: 
http://dx.doi.org/10.1111/wrr.12041
URI: 
Access Rights: 
Acesso restrito
Type: 
outro
Source:
http://repositorio.unesp.br/handle/11449/75268
Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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