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No evidence for association of the CD40, CD40L and BLYS polymorphisms, B-cell co-stimulatory molecules, with Brazilian endemic Plasmodium vivax malaria
  • Universidade Estadual Paulista (UNESP)
  • Faculdade de Medicina de São José do Rio Preto (FAMERP)
  • Universidade Federal do Sergipe
  • Ministério da Saúde
  • Faculdade SEAMA
  • 0035-9203
  • 1878-3503
Background: Plasmodium vivax is the most prevalent malaria species in Brazil. The parasite-host coevolutionary process can be viewed as an 'arms race', in which adaptive genetic changes in one are eventually matched by alterations in the other. Methods: Following the candidate gene approach we analyzed the CD40, CD40L and BLYS genes that participate in B-cell co-stimulation, for associations with P. vivax malaria. The study sample included 97 patients and 103 controls. We extracted DNA using the extraction and purification commercial kit and identified the following SNPs: 21C.T in the CD40 gene, 2726T.C in the CD40L gene and the 2871C.T in the BLyS gene using PCR-RFLP. We analyzed the genotype and allele frequencies by direct counting. We also compared the observed with the expected genotype frequencies using the Hardy-Weinberg equilibrium. Results: The allele and genotype frequencies for these SNPs did not differ statistically between patient and control groups. Gene-gene interactions were not observed between the CD40 and BLYS and between the CD40L and BLYS genes. Overall, the genes were in Hardy-Weinberg equilibrium. Significant differences were not observed among the frequencies of antibody responses against P. vivax sporozoite and erythrocytic antigens and the CD40 and BLYS genotypes. Conclusions: The results of this study show that, although the investigated CD40, CD40L and BLYS alleles differ functionally, this variation does not alter the functionality of the molecules in a way that would interfere in susceptibility to the disease. The variants of these genes may influence the clinical course rather than simply increase or decrease susceptibility. © Royal Society of Tropical Medicine and Hygiene 2013. All rights reserved.
Issue Date: 
Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 107, n. 6, p. 377-383, 2013.
Time Duration: 
  • BLYS
  • CD40
  • CD40L
  • Malaria
  • Plasmodium vivax
  • Single nucleotide polymorphism
  • B cell activating factor
  • CD40 antigen
  • CD40 ligand
  • DNA
  • erythrocyte antigen
  • allele
  • antibody response
  • B lymphocyte
  • comparative study
  • controlled study
  • disease association
  • DNA extraction
  • DNA purification
  • gene frequency
  • gene interaction
  • gene linkage disequilibrium
  • genotype
  • human
  • major clinical study
  • Plasmodium vivax malaria
  • single nucleotide polymorphism
  • sporozoite
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Appears in Collections:Artigos, TCCs, Teses e Dissertações da Unesp

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