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- Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa
- UMR-CNRS 5085, Université Paul Sabatier
- UMR-CNRS 5503, Université Paul Sabatier
- Universidade Estadual Paulista (UNESP)
- Universidade de São Paulo (USP)
- Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium(II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix. © 2013 Copyright Taylor and Francis Group, LLC.
- Biofouling, v. 29, n. 7, p. 775-788, 2013.
- bifunctional silica nanoparticles
- P. aeruginosa (PAO1) biofilms
- surface modification
- functional group
- microbial community
- particle size
- reaction kinetics
- Pseudomonas aeruginosa
- Acesso restrito
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