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- eIF5A binds to translational machinery components and affects translation in yeast
- Universidade Estadual Paulista (UNESP)
- Harvard Univ
- The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation. (c) 2006 Elsevier B.V. All rights reserved.
- Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 348, n. 4, p. 1358-1366, 2006.
- Elsevier B.V.
- protein synthesis
- Acesso restrito
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